PCR conditions for CAPS ALDH1
PCR conditions (see also general conditions):
Option 1:
35 cycles of:
94 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute
Primers:
B143 and B144
Cut PvuII
Option 2:
35 cycles of:
94 degrees 30 seconds
52 degrees 30 seconds
72 degrees 1 minute
Primers:
B144 and B229
Cut with HaeIII
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