PCR conditions for dCAPS Pt13 and AFLP Pt13
PCR conditions (see also general conditions):
For CAPS:
35 cycles of:
94 degrees 30 seconds
57 degrees 30 seconds
72 degrees 1 minute
Primers:
KH38 and KH39
For AFLP
45 cycles of:
96 degrees 15 seconds
50 degrees 30 seconds
72 degrees 1 minute
Primers:
Pt13F and Pt13R
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