PCR conditions for CAPS FLS

PCR conditions (see also general conditions):

Option 1:
40 cycles of:

94 degrees 30 seconds
54 degrees 30 seconds
72 degrees 1 minute

Primers:
B46 and B47

(see gel)

Option 2:
35 cycles of:

94 degrees 30 seconds
52 degrees 30 seconds
72 degrees 1 minute

Primers:
FLSex2F and FLSR2

Cut with BtsCI (see gel)

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