PCR conditions for CAPS IGS

PCR conditions (see also general conditions):

Option 1:
35 cycles of:

94 degrees 30 seconds
50 degrees 30 seconds
72 degrees 1 minute

Primers:
B273 and B280

Cut HaeIII

Option 2:
35 cycles of:

94 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute

Primers:
B273 and B274

Cut EcoRV

Option 3:
35 cycles of:

94 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute

Primers:
B273 and B274

Cut FspI

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