PCR conditions (see also general conditions):

Option 1: MYB-FL-5’CAPS

35 cycles of:
94 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute

Use primers B371 and B368

cut Tsp45I

Option 2: MYB-FL-3’CAPS

30 cycles of:
94 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute

Use primers B404 and B405

cut MnlI

Option 3: MYB-FL-secreta-CAPS

35 cycles of:
95 degrees 30 seconds
55 degrees 30 seconds
72 degrees 1 minute
Use primers B406 and B422

cut HpyCH4V