PCR conditions for CAPS MYBph3 prot3
PCR conditions (see also general conditions):
3'end:
35 cycles of:
94 degrees 30 seconds
52 degrees 30 seconds
72 degrees 1 minute
Use primers B81 and B82
cut HaeIII
or FspI
5'end:
35 cycles of:
94 degrees 30 seconds
52 degrees 30 seconds
72 degrees 1 minute
Use primers B55 and B56
cut RsaI
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