The research of our group was in the field of the regulation,
localization and metabolic engineering of sulfate assimilation
and glutathione synthesis. We used Arabidopsis thaliana, potato
and maize as plant material. Our special interest was in regulatory
aspects during stress situations like cold, heavy metal, herbicides
or air pollutants and during increased demand for the synthesis
of sulfur rich storage proteins. Our experiments showed that plants
are able to cope with different stress situations by increasing
there glutathione production. This tripeptide is involved in the
detoxification of heavy metals and herbicides by forming phytochelatins
and glutathione conjugates, respectively. Glutathione also protects
plants in conditions of oxidative stress. For increasing the production
of glutathione plants increase the flux through assimilatory sulfate
reduction for synthesising higher amounts of cysteine, one of the
amino acids of glutathione. Our analysis aimed at determining changes
in mRNA, enzyme protein, enzyme activity and fluxes under this
condition.
Herschbach, C; Schulte, M; von Ballmoos, P; Brunold, C; Rennenberg, H
Sulfate and Nitrate Assimilation in Leaves of Quercus ilex and
Quercus pubescens Grown Near Natural CO2 Springs in Central Italy in: L.J. De Kok et al. (eds.), Sulfur Metabolism in Plants, Proceedings of the International 237
Plant Sulfur Workshop: 237-248 [pdf]
2003
Hesse H, Trachsel N, Suter M, Kopriva S, von Ballmoos P, Rennenberg
H, Brunold C
Effect of glucose on assimilatory sulphate reduction in
Arabidopsis thaliana roots
Journal of Experimental Botany
54 (388): 1701-1709 [pdf]
2002
Schulte, M; von Ballmoos, P; Rennenberg, H; Herschbach, C
Life-long growth of Quercus ilex L. at natural CO2 springs acclimates sulphur,
nitrogen and carbohydrate metabolism of the progeny to elevated pCO (2)
Plant Cell Env. 25 (12): 1715-1727. [pdf]
Kopriva S; Suter M; von Ballmoos P; Hesse H; Krähenbühl
U; Rennenberg H; Brunold C: Interaction of sulfate assimilation with
carbon and nitrogen metabolism in Lemna minor. Plant Physiol.
130 1406-1413. [pdf]
Vauclare P; Kopriva S; Fell D; Suter M; Sticher L; von Ballmoos P;
Krähenbühl
U; den Camp RO; Brunold C: Flux control of sulphate assimilation in
Arabidopsis thaliana: adenosine 5 '-phosphosulphate reductase is
more susceptible than
ATP sulphurylase to negative control by thiols. Plant J 31 (6) 729-740.
Kopriva S; Büchert T; Fritz G; Suter M; Benda RD; Schunemann
V; Koprivova A; Schurmann P; Trautwein AX; Kroneck PMH; Brunold
C: The presence of an iron-sulfur cluster in adenosine 5 '-phosphosulfate
reductase separates organisms utilizing adenosine 5 '-phosphosulfate
and phosphoadenosine 5 '-phosphosulfate for sulfate assimilation.
J
Biol Chem 277 (24) 21786-21791.
2001
Kopriva, S; Büchert, T; Fritz, G; Suter, M; Weber, M; Benda,
R; Schaller, J; Feller, U; Schürmann, P; Schünemann, V;
Trautwein, AX; Kroneck, PMH; Brunold, C: Plant adenosine 5 '-phospho
sulfate reductase is a novel iron-sulfur protein. Journal of Biological
Chemistry, 276: (46) 42881-42886.
Kocsy, G; von Ballmoos, P; Ruegsegger, A; Szalai, G; Galiba,
G; Brunold, C.: Increasing the glutathione content in a chilling-sensitive
maize genotype using safeners increased protection against chilling-induced
injury. Plant Physiol., 127: (3) 1147-1156. [pdf]
Kocsy, G; Galiba, G; Brunold, C. Role of glutathione in adaptation
and signalling during chilling and cold acclimation in plants.
Physiol. Plant. 113: (2) 158-164.
Koprivova, A; Melzer, M; von Ballmoos, P; Mandel, T; Brunold,
C; Kopriva, S: Assimilatory sulfate reduction in C-3, C-3-C-4,
and C-4 species of Flaveria. Plant Physiol., 127: (2) 543-550.
[pdf]
Kopriva, S., Jones, S., Koprivova, A., Suter, M., von Ballmoos,
P., Brander, K., Flückiger, J., Brunold, C.: Influence
of Chilling Stress on the Intercellular distribution of Assimilatory
Sulfate
Reduction and Thiols in Zea mays. Plant Biol. 3, 24-31.
2000
Brunold, C., Rennenberg, H., De Kok, L.J., Stulen, I., Davidian,
J.-C. (eds.): Sulfur Nutrition and Sulfur Assimilation in Higher
Plants. Molecular, Biochemical and Physiological Aspects. Paul Haupt
Publishers. ISBN 3-258-06239-0
Harms, K., von Ballmoos, P., Brunold, C., Höfgen, R. and
Hesse, H.: Expression of a bacterial serine acetyltransferase in
transgenic potato plants leads to increased levels of cysteine
and glutathione. The Plant Journal, 22 (4), 335-343.
Kocsy, G., von Ballmoos, P., Suter, M., Rüegsegger, A.,
Galli, U., Szalai, G., Galiba, G. and Brunold, C.: Inhibition
of glutathione synthesis reduces chilling tolerance in maize. Planta,
211 (4), 528-536.
Weber, M; Suter, M; Brunold, C; Kopriva, S: Sulfate assimilation
in higher plants - Characterization of a stable intermediate
in the adenosine 5 '-phosphosulfate reductase reaction. European
Journal
of Biochemistry, 267: (12) 3647-3653.
Koprivova, A., Suter, M., Op den Camp, R., Brunold, C., Kopriva,
S.: Regulation of Sulfate Assimilation by Nitrogen in Arabidopsis.
Plant Physiology, 122, 737-746. [pdf]
Kopriva, S., Koprivova, A. and Süss K.: Identification,
Cloning, and Properties of Cytosolic D-Ribulose-5-Phosphate
3-Epimerase from Higher Plants. Journal of Biological Chemistry,
275 (2), 1294-1299.
Suter, M., von Ballmoos, P., Kopriva, S., Op den Camp, R., Schaller,
J., Kuhlemeier, C., Schürmann, P. and Brunold, C.:
Adenosine 5'-phosphosulfate sulfotransferase and adenosine
reductase are identical
enzymes. Journal of Biological Chemistry, 275 (2), 930-936.
1999
Ammann, M., Siegwolf, R., Pichlmayer, M., Suter, M., Saurer, M.,
Brunold, C.: Estimating the uptake of traffic derived NO2 from 15N
abundance in Norway spruce needles. Oecologia, 118, 124-131.
Kopriva, S., Muheim, R., Koprivova, A., Trachsel, N., Catalano,
C., Suter, M. and Brunold, (1999). Light regulation of assimilatory
sulfate reduction in Arabidopsis thaliana. The Plant Journal, 20
(1), 37-44.
1998
Tadege, M., Bucher, M., Stähli, W., Suter, M., Dupuis, I.,
Kuhlemeier, C.: Activation of plant defense responses and sugar efflux
by expression of pyruvate decarboxylase in potato leaves. Plant J.
16 (6), 661-671.
Burgener, M., Suter, M., Jones, S., Brunold, C.: Cyst (e)ine is
the transport metabolite of assimilated sulfur from bundle-sheath
to mesophyll cells in maize leaves. Plant Physiol., 116, 1315-1322. [pdf]
von Ballmoos, P., Ammann, M., Egger, A., Suter, M.,
Brunold, C.: NO2-induced nitrate reductase activity in needles of
Norway spruce
(Picea abies) under laboratory and field conditions. Physiol.
Plant., 102, 596-604.
1997
Kocsy, G., Owttrim, G., Brander, K., Brunold, C.: Effect of chilling
on the diurnal rhythm of enzymes involved in protection against oxidative
stress in a chilling-tolerant and a chilling-sensitive maize genotype.
Physiol. Plant., 99, 249-254.
Brunold, C. and Rennenberg, H.: Regulation of sulfur metabolism
in plants: First molecular approaches. Progress in Botany 58: 164
- 186.
Brunold, C., von Ballmoos, P., Nussbaum, S., Ammann, M.,
Stalder, M., Schlunegger, U.P., and Gfeller, H.: Uptake and Distribution
of NO2 in Norway Spruce (Picea abies [L.]
Karst.). In: Biosphere-Atmosphere Exchange of Pollutants and Trace
Substances, S. Slanina ed., Springer,
pp. 413-419.
1996
Fürtig, K., Rüegsegger, A., Brunold, C. and Brändle,
R.: Sulphide utilisation and injuries in hypoxic roots and rhizomes
of common reed (Phragmites australis). Folia Geobotanica et Phytotaxonomica
31: 143 - 151.
Galli, U, Schüepp, H., Brunold, C.: Thiols in cadmium and
copper-treated maize (Zea mays L.). Planta 198, 139 - 143.
Kocsy, G., Brunner, M., Rüegsegger, A., Stamp, P., Brunold,
C.: Glutathione synthesis in maize genotypes with different sensitivities
to chilling. Planta 198: 365 - 370.
Balsiger, P., Defila, R., Di Giulio, A.: Oekologie und Interdisziplinarität
- eine Beziehung mit Zukunft? Wissenschaftsforschung zur Verbesserung
der fachübergreifenden Zusammenarbeit. Themenhefte. Basel,
Boston, Berlin, Birkhäuser Verlag.
Brunold, C., Rüegsegger, A. and Brändle R., Hrsg.:
Stress bei Pflanzen, UTB, P. Haupt-Verlag, Bern-Stuttgart-Wien, 407
pp.
Institute of Plant Sciences
