Universitaet Bern Institute of Plant Sciences swissuniversity.ch  
Plant Sciences

Plant Stress



Prof. em. Dr. Chr. Brunold

e-mail Christian.Brunold@ips.unibe.ch
internet http://www.botany.unibe.ch/emeriti/sulfur.php

The research of our group was in the field of the regulation, localization and metabolic engineering of sulfate assimilation and glutathione synthesis. We used Arabidopsis thaliana, potato and maize as plant material. Our special interest was in regulatory aspects during stress situations like cold, heavy metal, herbicides or air pollutants and during increased demand for the synthesis of sulfur rich storage proteins. Our experiments showed that plants are able to cope with different stress situations by increasing there glutathione production. This tripeptide is involved in the detoxification of heavy metals and herbicides by forming phytochelatins and glutathione conjugates, respectively. Glutathione also protects plants in conditions of oxidative stress. For increasing the production of glutathione plants increase the flux through assimilatory sulfate reduction for synthesising higher amounts of cysteine, one of the amino acids of glutathione. Our analysis aimed at determining changes in mRNA, enzyme protein, enzyme activity and fluxes under this condition.
  • Herschbach, C; Schulte, M; von Ballmoos, P; Brunold, C; Rennenberg, H
    Sulfate and Nitrate Assimilation in Leaves of Quercus ilex and Quercus pubescens Grown Near Natural CO2 Springs in Central Italy
    in: L.J. De Kok et al. (eds.), Sulfur Metabolism in Plants, Proceedings of the International 237 Plant Sulfur Workshop: 237-248 [pdf]

  • Hesse H, Trachsel N, Suter M, Kopriva S, von Ballmoos P, Rennenberg H, Brunold C
    Effect of glucose on assimilatory sulphate reduction in Arabidopsis thaliana roots
    Journal of Experimental Botany
    54 (388): 1701-1709 [pdf]

  • Schulte, M; von Ballmoos, P; Rennenberg, H; Herschbach, C
    Life-long growth of Quercus ilex L. at natural CO2 springs acclimates sulphur, nitrogen and carbohydrate metabolism of the progeny to elevated pCO (2)
    Plant Cell Env. 25 (12): 1715-1727. [pdf]

  • Kopriva S; Suter M; von Ballmoos P; Hesse H; Krähenbühl U; Rennenberg H; Brunold C: Interaction of sulfate assimilation with carbon and nitrogen metabolism in Lemna minor. Plant Physiol. 130 1406-1413. [pdf]

  • Vauclare P; Kopriva S; Fell D; Suter M; Sticher L; von Ballmoos P; Krähenbühl U; den Camp RO; Brunold C: Flux control of sulphate assimilation in Arabidopsis thaliana: adenosine 5 '-phosphosulphate reductase is more susceptible than ATP sulphurylase to negative control by thiols. Plant J 31 (6) 729-740.

  • Kopriva S; Büchert T; Fritz G; Suter M; Benda RD; Schunemann V; Koprivova A; Schurmann P; Trautwein AX; Kroneck PMH; Brunold C: The presence of an iron-sulfur cluster in adenosine 5 '-phosphosulfate reductase separates organisms utilizing adenosine 5 '-phosphosulfate and phosphoadenosine 5 '-phosphosulfate for sulfate assimilation. J Biol Chem 277 (24) 21786-21791.

  • Kopriva, S; Büchert, T; Fritz, G; Suter, M; Weber, M; Benda, R; Schaller, J; Feller, U; Schürmann, P; Schünemann, V; Trautwein, AX; Kroneck, PMH; Brunold, C: Plant adenosine 5 '-phospho sulfate reductase is a novel iron-sulfur protein. Journal of Biological Chemistry, 276: (46) 42881-42886.

  • Kocsy, G; von Ballmoos, P; Ruegsegger, A; Szalai, G; Galiba, G; Brunold, C.: Increasing the glutathione content in a chilling-sensitive maize genotype using safeners increased protection against chilling-induced injury. Plant Physiol., 127: (3) 1147-1156. [pdf]

  • Kocsy, G; Galiba, G; Brunold, C. Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol. Plant. 113: (2) 158-164.

  • Koprivova, A; Melzer, M; von Ballmoos, P; Mandel, T; Brunold, C; Kopriva, S: Assimilatory sulfate reduction in C-3, C-3-C-4, and C-4 species of Flaveria. Plant Physiol., 127: (2) 543-550. [pdf]

  • Kopriva, S., Jones, S., Koprivova, A., Suter, M., von Ballmoos, P., Brander, K., Flückiger, J., Brunold, C.: Influence of Chilling Stress on the Intercellular distribution of Assimilatory Sulfate Reduction and Thiols in Zea mays. Plant Biol. 3, 24-31.

  • Brunold, C., Rennenberg, H., De Kok, L.J., Stulen, I., Davidian, J.-C. (eds.): Sulfur Nutrition and Sulfur Assimilation in Higher Plants. Molecular, Biochemical and Physiological Aspects. Paul Haupt Publishers. ISBN 3-258-06239-0

  • Harms, K., von Ballmoos, P., Brunold, C., Höfgen, R. and Hesse, H.: Expression of a bacterial serine acetyltransferase in transgenic potato plants leads to increased levels of cysteine and glutathione. The Plant Journal, 22 (4), 335-343.

  • Kocsy, G., von Ballmoos, P., Suter, M., Rüegsegger, A., Galli, U., Szalai, G., Galiba, G. and Brunold, C.: Inhibition of glutathione synthesis reduces chilling tolerance in maize. Planta, 211 (4), 528-536.

  • Weber, M; Suter, M; Brunold, C; Kopriva, S: Sulfate assimilation in higher plants - Characterization of a stable intermediate in the adenosine 5 '-phosphosulfate reductase reaction. European Journal of Biochemistry, 267: (12) 3647-3653.

  • Koprivova, A., Suter, M., Op den Camp, R., Brunold, C., Kopriva, S.: Regulation of Sulfate Assimilation by Nitrogen in Arabidopsis. Plant Physiology, 122, 737-746. [pdf]

  • Kopriva, S., Koprivova, A. and Süss K.: Identification, Cloning, and Properties of Cytosolic D-Ribulose-5-Phosphate 3-Epimerase from Higher Plants. Journal of Biological Chemistry, 275 (2), 1294-1299.

  • Suter, M., von Ballmoos, P., Kopriva, S., Op den Camp, R., Schaller, J., Kuhlemeier, C., Schürmann, P. and Brunold, C.: Adenosine 5'-phosphosulfate sulfotransferase and adenosine reductase are identical enzymes. Journal of Biological Chemistry, 275 (2), 930-936.

  • Ammann, M., Siegwolf, R., Pichlmayer, M., Suter, M., Saurer, M., Brunold, C.: Estimating the uptake of traffic derived NO2 from 15N abundance in Norway spruce needles. Oecologia, 118, 124-131.

  • Kopriva, S., Muheim, R., Koprivova, A., Trachsel, N., Catalano, C., Suter, M. and Brunold, (1999). Light regulation of assimilatory sulfate reduction in Arabidopsis thaliana. The Plant Journal, 20 (1), 37-44.

  • Tadege, M., Bucher, M., Stähli, W., Suter, M., Dupuis, I., Kuhlemeier, C.: Activation of plant defense responses and sugar efflux by expression of pyruvate decarboxylase in potato leaves. Plant J. 16 (6), 661-671.

  • Burgener, M., Suter, M., Jones, S., Brunold, C.: Cyst (e)ine is the transport metabolite of assimilated sulfur from bundle-sheath to mesophyll cells in maize leaves. Plant Physiol., 116, 1315-1322. [pdf]

  • von Ballmoos, P., Ammann, M., Egger, A., Suter, M., Brunold, C.: NO2-induced nitrate reductase activity in needles of Norway spruce (Picea abies) under laboratory and field conditions. Physiol. Plant., 102, 596-604.

  • Kocsy, G., Owttrim, G., Brander, K., Brunold, C.: Effect of chilling on the diurnal rhythm of enzymes involved in protection against oxidative stress in a chilling-tolerant and a chilling-sensitive maize genotype. Physiol. Plant., 99, 249-254.

  • Brunold, C. and Rennenberg, H.: Regulation of sulfur metabolism in plants: First molecular approaches. Progress in Botany 58: 164 - 186.

  • Brunold, C., von Ballmoos, P., Nussbaum, S., Ammann, M., Stalder, M., Schlunegger, U.P., and Gfeller, H.: Uptake and Distribution of NO2 in Norway Spruce (Picea abies [L.] Karst.). In: Biosphere-Atmosphere Exchange of Pollutants and Trace Substances, S. Slanina ed., Springer, pp. 413-419.
  • Fürtig, K., Rüegsegger, A., Brunold, C. and Brändle, R.: Sulphide utilisation and injuries in hypoxic roots and rhizomes of common reed (Phragmites australis). Folia Geobotanica et Phytotaxonomica 31: 143 - 151.

  • Galli, U, Schüepp, H., Brunold, C.: Thiols in cadmium and copper-treated maize (Zea mays L.). Planta 198, 139 - 143.

  • Kocsy, G., Brunner, M., Rüegsegger, A., Stamp, P., Brunold, C.: Glutathione synthesis in maize genotypes with different sensitivities to chilling. Planta 198: 365 - 370.

  • Balsiger, P., Defila, R., Di Giulio, A.: Oekologie und Interdisziplinarität - eine Beziehung mit Zukunft? Wissenschaftsforschung zur Verbesserung der fachübergreifenden Zusammenarbeit. Themenhefte. Basel, Boston, Berlin, Birkhäuser Verlag.

  • Brunold, C., Rüegsegger, A. and Brändle R., Hrsg.: Stress bei Pflanzen, UTB, P. Haupt-Verlag, Bern-Stuttgart-Wien, 407 pp.

List of publications